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Biography:
Dr. Sadia Sattar has completed her PhD in Biochemistry/ Molecular biology from Massey University New Zealand on HEC merit scholarship in 2014. she has a strong ambition to utilize her research and teaching expertise for the betterment of institution she serves and the country in general. Her area of strength includes
1. Bacteriophage Isolation and Typing from diverse environmental samples.
2. Computational and phylogenetic analysis of bacteriophage and viral genomes.
3. She is also working on cloning and expression of lysins and use of bacteriophages for biofilm reduction.
Research and Teaching skills She is an enthusiastic researcher and have a great passion for teaching. She has been working as Assistant Professor at one of the top institutes in Pakistan (CIIT) since June 2014. I have taught Microbiology and immunology, Principles of virology, and Principles of immunology to bachelors students. she has also developed and taught Vaccinology course to MS students.. She encourages individual learning and development of critical analysis skills in students.
Research skills.
Her passion for science and research was fueled during her MPhil. research project where she developed and tested potential of vaccines developed from three field isolates of Avian Influenza Virus H7N3.She conducted an experiment for almost a year that included injecting handling and bleeding chicken model. It also included ELISA, Hemagglutination test, Hemagglutination inhibition test , Virus neutralization as well as egg inoculation and large-scale production of these field isolates. Later she joined the same lab as research associate and worked on large scale production, lyophilization, storage and typing of Avian Influenza H7N3, H9N2 as well as H5N1 (the Bird Flu strains). This provided her with hands on training in basic virus typing assays.
Later she went for her PhD in July 2009 on HEC merit scholarship. During her PhD she worked on Filamentous Bacteriophages (viruses that eat bacteria). she worked on optimizing their production, detection, and purification (for vaccine applications) of nano phage particle (50nm). Later she tested these particles for use in diagnostics in lateral flow dip stick device as well as probes for vaccine antigen delivery. During the first phase she had hands on experience with techniques such as phage growth, differential PEG purification , CsCl2 gradient purifications, FITC labelling, SDS PAGE, Southern and Northern blotting, Protein purification using MBP and Electron microscopy, preparation of dipsticks spraying and testing their ability. In second phase she developed nanoparticles expressing FnB (Fibronectin Binding Peptide) and tested them as antigen carriers. she has through experience of ethical framework and considerations required for animal model handling.She had thorough experience in handling mouse model to test the efficacy of nanophage as vaccine carries.
She started working at CUI in 2014, and had to figure out and outline and direction of her work that can be of benefit to her people and society in general. Since antibiotic resistance is a big problem in Pakistan, she started working to find alternative cures using bacteriophages for control of Multiple Drug Resistant pathogenic bacterial species of animal and human origin such as Salmonella, E.coli, A. baumannii, E. faecalis and K. pneumoniea (Please see thesis supervision and publication list for reference) She has isolated bacteriophages against several bacterial strains and has have published research in international peer review journals (Nature Portfolio, MDPI and ASM press). It took her some time to develop a platform which can yield good data and she can produce not only good publications but some bacteriophage cocktails for application in industry.
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